Original Source: https://kendricklabsinc.wordpress.com/2023/04/18/2d-gel-electrophoresis-understanding-the-technique-and-applications/

2D gel electrophoresis is a powerful technique used for the separation and analysis of complex protein mixtures. It involves the separation of proteins based on their charge and molecular weight, enabling the detection of hundreds to thousands of proteins in a single analysis.

The technique involves two separate steps: the first dimension separates proteins based on their charge using isoelectric focusing (IEF), and the second dimension separates proteins based on their molecular weight using SDS-PAGE.

2D gel electrophoresis has numerous applications, including the identification of biomarkers, protein expression profiling, and the study of post-translational modifications such as phosphorylation and glycosylation. It has been used in a variety of fields, including cancer research, drug discovery, and proteomics.

Despite its many benefits, 2D gel electrophoresis has some limitations, including low sensitivity for low abundance proteins and poor reproducibility between gels. However, these limitations can be overcome by the use of advanced detection methods such as fluorescent staining, mass spectrometry, and western blotting.

Kendrick Labs, Inc is a leading provider of 2D gel electrophoresis services, offering high-quality sample analysis using state-of-the-art equipment and techniques. Our team of experienced scientists can provide customized solutions to meet your specific research needs, from protein identification and quantification to post-translational modification analysis.

In addition to our 2D gel electrophoresis services, Kendrick Labs offers a range of other protein analysis services, including protein sequencing, peptide mapping, and protein-protein interaction analysis. We are committed to providing our clients with accurate and reliable data, delivered in a timely and cost-effective manner.

Whether you are a researcher in academia or industry, Kendrick Labs can help you achieve your research goals with our high-quality protein analysis services. Contact us today to learn more about our services and how we can support your research needs.